Evaluation of Rat Reference Genes for Improved Quantitative Gene Expression Analysis
Gene Expression
Jenny Hong Cai, MD, Medical Development Team, Emerging Market Business Unit Pfizer, Inc.
Abstract
Real-time PCR is an important technique in assessing gene expressions and has been broadly adopted in many laboratories for gene expression profiling research. It is widely agreed that the normalized data are required for accurate estimates of gene expression analysis and the
internal reference genes should be used to establish the comparisons. In order to generate meaningful results, the selection of the internal reference genes (normally stably expressed genes) is essential to the success of accurately interpreting the data. There are a number of studies evaluating the selection of internal control genes, however, many of them are derived from human research. With increased gene expression profiling in preclinical research and toxicogenomics, the needs in identifying the appropriate control genes in animal models have emerged. Rat is one commonly used species in preclinical research and the evaluation
of commonly used control genes among various tissues in rat for gene expression analysis are becoming important and can provide values in understanding and bridging the hypothesis of gene expression in human research. This paper describes an evaluation of commonly used housekeeping genes among various rat tissues for gene expression analysis in quantitative real-time PCR.
This article was published in the February/March 2010 issue of International Drug Discovery, Volume 5, Issue 1, on pgs. 22-26.
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